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The wild-type TsPOx or its mutants was supplemented at the dosage of 0.25, 0.50, 0.75 or 1.00 nkat/g flour.
To investigate the effect of POx on gluten agglomeration, 0.25, 0.5, 0.75, and 1.0 nkat/g flour of wild-type TsPOx or its mutants was supplemented.
This energy-based criterion for selection of mutants was supplemented by considering conservation of the receptor sequences in various species to avoid mutating the most preserved residues that may carry significant structural or functional role.
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The cell culture medium for ISE6 cells infected with the A. marginale omp10:: himutantutant was supplemented with spectinomycin (Sigma Aldrich) and streptomycin (Sigma Aldrich) to a final concentration of 50 μg/ml each.
For the selection and maintenance of mutants, BG11 medium was supplemented with kanamycin (Km, 10 600 µg ml−1).
The catalytically inactive mutant MMP-9(E402A) [38] was supplemented at a final concentration of 0.01 mg/ml (120 nM) to another sample that contained collagen and MMP-8 at the indicated concentrations.
To allow growth of asd mutant strains, the medium was supplemented with 50 μg/ml meso-diaminopimelic acid.
This group of genes was supplemented with developmental mutants identified using the mariner-based transposable element magellan-4 [14], which was used previously to identify motility genes in M. xanthus [15] [17].
Normal growth of the mutant was only restored when CDM was supplemented with potassium at high concentration.
As shown in Fig. 6a, the severely inhibited primary root growth was observed in the stt3a-2 mutants when the high dose of salinity was supplemented in the medium.
These mutant spores, however, germinated efficiently when inosine was supplemented with alanine.
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