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A "triple mutant" construct containing all three changes was created from wild-type BCR-ABL by a series of standard subcloning steps in a modified pGEM5z vector (Promega, Madison, WI, USA).
A scatter plot of log2-fold changes was created (Fig. 1), and the correlation coefficient between the gene dataset and each contig group was calculated (Table 4).
Then the signals were aligned to the annotation library and a spreadsheet containing gene symbols, p-values and expression fold changes was created.
To test the hypothesis that the diet intervention would have a measurable overall effect on the metabolic profile, a univariate score that summarized the metabolic changes was created.
A tomographic image of impedance changes was created every 100 ms (10 Hz) by the application of an electrical current of 5 mA and 50 kHz to the electrodes.
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Sir David said the funding situation, coupled with the NHS changes, was creating a "new world" for the health service.
A sweeping set of cultural and technological changes was creating digitally-savvy learners "who have experienced increasingly sophisticated video games and have spent hours with such excellent simulations as Sim City and Flight Simulator".
The results show that land use changes are created by systematic transitions, with or without gross variations, and also by casuistic processes associated with an increase in artificialised areas and the growth of the continuous urban fabric.
The first classifications of IPCL changes were created for the esophagus [ 6] and the oral cavity [ 10].
A log of any changes is created along with relevant time and date stamps and a report generated.
Furthermore, most of the morphological changes were created by deep invasion of the cancers beyond the mucosal layer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com