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In the presence of sorbitol the double mutants can grow at 30°C but behaved as cdc48-3 atone at 32°C (Fig. 8B).
According to Burgard et al. [ 5], these mutants can grow anaerobically on glucose by producing lactate.
Hopanoid-deficit mutants can grow at optimized laboratory conditions; however, the mutants act differently under stress conditions.
This results in a markedly different outcome when the cells are incubated in HL5 medium: mutants can grow but the wildtype cannot.
Taken together, these results demonstrate that, although the FPV mutants can grow to high titres in the allantoic cavity of chicken eggs, they otherwise behave as typical LPAIV which fail to infect and to rapidly kill chicken embryos.
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The rationale behind this is as follows: if there was outgrowth of a particular mutant in presence of a drug with some concentration, then it is likely that this mutant can grow in any lower concentration of that drug.
However, the pnfA mutant can grow on nitrogen-free semisolid medium, indicating that it has a Nif+ phenotype (data not shown).
sphaeroides uses the EMCoA pathway (and not the citramalate cycle) under photoheterotrophic conditions because only this pathway can explain the fact that a Rubisco mutant can grow on acetate.
The aroD mutant can grow on the peptone-based media used in C. elegans studies, so the media must be able to provide either all the essential aromatic compounds needed for growth or the relevant precursors.
To solve the problem, a novel atmospheric and room temperature plasma mutation method was employed to generate mutants which can grow in the defined medium.
Homozygous mutant (y11/y11) plants of the mutant T219H can grow through the seedling stage, but do not survive to set seed.
Related(20)
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