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Exact(11)
Concentration of mutant was calculated from triplicate using the Bio-Rad QuantaSoft platform.
Theoretical % mutant was calculated from TaqMan measured concentrations of mutant and wild-type template.
Percent mutant was calculated as follows: 2 We calculated the average and standard deviation across triplicates as weighted by the number of detected droplets in each well.
The mean sleep value for each mutant was calculated by assaying sleep in 4 8 individual flies in the presence of 12 hr L-12 hr D cycles.
Differential expression in poly(A) selected libraries between wild-type and tdp-1 ok803 tdp-1 ok803 calculated using the Bioconductor package edgeR (release 2.12) (Robinson et al, 2010).
Enrichment or depletion of the mutant was calculated by dividing this ratio by the ratio obtained for the wild type for the same sample for a relative in vivo fitness of the mutant.
Similar(49)
Expression levels of target genes in the ashh2 mutant were calculated relative to wt levels with normalization to ACTIN2, using the formula RE = EtargetΔCPtarget (wt- ashh2)]/Eactin[ ΔCPactin (wt – ashh2)], where target represents the target gene analyzed.
Statistical differences between wild-type and mutant were calculated by using the Mann-Whitney nonparametric U test.
Significant differences between the A. marginale wild-type and omp10::himutantutant were calculated by Student's t test (P < 0.05), comparing ∆Ct values (target gene- reference gene) of the omp10::himutantutand and the wild-type.
Relative ligand-binding capacity (represented by binding %) to different mutants was calculated by the MS response of the ligand released from a specific mutant divided by the MS response of the total ligand present in the incubation sample.
The wt TSHR was set at 100% and receptor expression of the mutants was calculated according to this.
Related(20)
line was calculated
mutation was calculated
act was calculated
loi was calculated
variant was calculated
mutations was calculated
variants was calculated
transformants was calculated
mutant was analyzed
mutant was derived
mutant was characterized
mutant was used
mutant was termed
mutant was shortened
mutant was recruited
mutant was sequenced
mutant was conducted
mutant was generated
mutant was found
mutant was isolated
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