Sentence examples for mutant was conducted from inspiring English sources

Temperature sensitive KOG1 mutant was conducted by mutagenic PCR.

Each experiment with wild-type BAP1 or F170I mutant was conducted twice.

However, in contrast to these results, when complementation of the P. grandiflora dod mutant was conducted with PgDOD, only single cell foci occurred [ 11].

Reconstitution of the purified wild type or E325A mutant was conducted with E. coli total phospholipids (Avanti Polar Lipids, Alabaster, AL) by using octyl glucoside dilution, followed by one cycle of freeze−thaw sonication (18, 19).

Site-directed mutagenesis to obtain the mutant genes (W7F for all single-cysteine mutants, followed by replacement of K77, K102, Y103, E105, F106, I111, M131, L135, F138, R139, K140, Y146, or K147 with cysteine, or S108C/L135C for the double-cysteine mutant) was conducted using the QuikChange Site-Directed Mutagenesis Kit (Stratagene).

Spin labeling to introduce the nitroxide side chain designated R1 and purification of each mutant were conducted on an immunoaffinity column as described previously.

As an extension of the WT studies delineated above, further studies on the I260Q mutant were conducted in the interest of kinetic comparison.

A pilot study of the feeding assay using wild-type and four eat mutants was conducted to determine the number of experimental replicates required for optimal statistical power.

An expression analysis of the genes in the lov1-4 mutants was conducted to confirm that LOV1 affects the expressions of CO and FT but not GI.

Site-specific mutagenesis to obtain ComFB mutants was conducted using standard protocols using Pfu polymerase and two oligonucleotides for each mutation (oTS387/oTS388; oTS387/oTS388; oTS387/oTS388).

Screening for atocd-2/p5cs1-4 atocd-2/p5cs1-4 atocd-2/p5cs1-4ing primers given in Adoublenal file 1: Table S2 and those reported previously for p5cs1-4[ 14].