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Each mutant was analyzed in duplicate in each assay, and each experiment was performed in triplicate.
Three mutants with different start codon of nosM were constructed, and nosiheptide production of each mutant was analyzed and compared.
Additionally, the transcriptome of this mutant was analyzed using RNA-seq in order to examine rearrangements of the metabolism during cultivation.
The relative expression level of each gene in WT and mutant was analyzed by qPCR and normalized using the OsActin as an internal control.
TO ultrastructure in the T192 mutant was analyzed by transmission electron microscopy (Fig. 5).
Growth of the Δafoch1 mutant was analyzed under these and additional stress conditions.
Similar(37)
Differences between the WT and each lse mutant were analyzed by Student's t-test.
Next, the reaction kinetics and biochemical properties of the mutant were analyzed.
The relative expression levels of the tri genes in the Δtri3 mutant were analyzed and compared with those of the wild-strain 0248.
In these experiments ten IAP alleles for each mutant were analyzed for the presence of methylated CpGs.
Single-cysteine substituted mutants on the background of the basal mutant were analyzed by rhodopsin chromophore formation, bleaching behavior, and Meta II decay.
More suggestions(15)
mutations was analyzed
transformants was analyzed
variants was analyzed
mutant was derived
mutant was caused
mutant was transformed
mutant was characterized
mutant was used
mutant were analyzed
mutant was shown
mutant was shortened
mutant was sequenced
mutant was identified
mutant was prepared
mutant was enhanced
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