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Interestingly, some genes related to anaerobic respiration were up-regulated in the mutant in relation to the wild-type control.

Furthermore until reach OD600 of 0.2 under anaerobiosis, there was no obvious delay in growth rate of the Δ fnr mutant in relation to the wild-type strain (See Additional file 1: Figure S1).

Moreover, it was found that for a majority of the COGs (K, M, O, G, C, T, V, P, I, H, R, S and that with hypothetical functions) a significantly larger number of the genes were up-regulated than down-regulated in the rosR mutant in relation to the parental strain (Fig.  1b).

Results indicated that "thick stem" mutant in relation to control shows significant (P < 0.01 to 0.001) enhancement of plant height, total number of branches per plant, total capsules per plant, seeds per capsule, seed yield per plant, and fibre yield per plant.

For this purpose, we first computed the relative expression level of genes in terms of fold-change (FC) for the hrpX mutant in relation to the wild-type strain, along with p-values to denote the statistical significance and false discovery rate (FDR), for having a good control over the false positives rate.

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The Cys to Ser mutations in Rli1 and the impact of these mutants in relation to oxygen level is interesting.

The comparison of genes differentially expressed in different mutants in relation to the wild-type strain revealed a high degree of coincidence, since most of the identified genes showed differential expression in more than one mutant.

However, it is unclear which of these are attributed to ULK3 and which are involved in abscission checkpoint signaling, complicating interpretation of results with the 4SA/E mutants in relation to ULK3.

We therefore assessed the distribution of mutations and their cooccurrence before and after this genetic bottleneck, as well as the evolution of these mutants in relation to the appearance of the two CD8+ T-cell responses identified within this region.

In this system, for INH-resistant strains, it may be prudent to perform selections as a two-step process, i.e., growth in broth at 39°C to eliminate plasmid replication followed by selection in solid medium containing hygromycin to increase the yield of double crossover mutants in relation to spontaneous hygromycin-resistant strains.

Here, we present a systematic analysis of S. pombe Mediator deletion mutants in relation to heterochromatin, and we identify roles played by the Med8-Med18-Med20 submodule in the transcriptional regulation of centromeric repeats and thus in heterochromatin formation, centromere function and chromosome segregation.

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