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Similarly, we constructed an avrBsT mutant in strain CFBP4834 of X. axonopodis pv.
Phenotypic analysis of the lac1Δ mutant in strain R265 revealed a severe melanin production defect on all melanin-inducing media (Figure 5A).
An lrgAB mutation was generated in the UAMS-1 background using a deletion plasmid previously used to make the lrgAB mutant in strain RN6390 [19].
Genes with a single disabling mutation in the sequenced strain are annotated as coding genes and marked as "mutant in strain" (see section above).
Such genes are classified as "polymorphic pseudogenes"; they are also annotated as "mutant in strain," with a gene model comment explaining their nature.
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To explore the function of the Lsp proteins, we generated seven in-frame deletion mutants in strain OH11 (Additional file 1: Tables S1, S2), including three having a single mutation (Δlsp1, Δlsp2 and Δlsp3), three containing double mutations (Δlsp12, Δlsp13 and Δlsp23), and one triple mutation (Δlsp123).
Derivatives of the suicide pID701t plasmid were used to construct disruptive mutants in strain ST556 by insertion duplication essentially as described [11].
Intriguingly, we observed an increase in UV-induced mutants in strains expressing pDinB+ but not pDinB(D103N) (compare columns NFZ or MMS with UV for pDinB+ in Fig. 7A).
We recently reported the successful construction of high activity prfA* isogenic mutants in strains containing promoterless copies of the genes encoding β-glucuronidase (gus) and neomycin resistance (neo) located in the chromosome downstream of the PrfA-dependent gene actA [41].
The oga-1 ok1207) oga-1 ok12071474) mutands in strains ET434 and ET435 were ogt-1 ok1474 ogt-1 ok1474t N2 in our laboratory with the mutantsns followed by PCR.
Also, we failed to demonstrate any change in the toxicity induced by the m8 mutant in this strain.
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