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Secondary, a reduction of available nitrogen slightly enlarged the size of the mutant in comparison to the corresponding genetic background.
The catalytic nucleophile residue of PoAbf was identified by site-directed mutagenesis and analysis of reactivity of the mutant in comparison to the wild-type enzyme.
This is further evidenced from the increase in fluorescence intensity for this mutant in comparison to the WT (Figure 4).
The inter-helical angle between α2 and α3 helices is 33° in the mutant in comparison to 51° in the WT protein structure (Figures 6C and D).
Kinetic analysis of soluble mutant ACE as well as membrane bound mutant in comparison with WT ACE using two widely used ACE substrates showed a very unexpected result.
In this study, a rsmB mutant Ech148 was constructed, and a reduced amount of hrpL mRNA was observed in this mutant in comparison with 3937 (Fig. 4).
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The differentiating Raman features are enhanced by preprocessing the data, which reduces the variability among the spectra from the same group Fig. 5 PC-LDA scores plot of 2- and 12 days old flies with (left) upheld 1 (up 1 ) mutation in comparison with control flies CS; (right) upheld 101 (up 101 ) mutants in comparison with control flies CS.
Further insights into the nature of the barrier to ROL and the molecular mechanism of inducible barrier formation could be achieved from the characterization of suberin or lignin formation-affected rice mutants in comparison with the respective wild types (Ranathunge et al. 2011).
We assessed lipid stores in all of the three mutants in comparison to wild type.
Thus, water transport is impaired in esk1 mutants in comparison to wild type and probably induces the low evapo-transpiration and the reduced RWC observed in the mutants.
An enhanced O-GlcNAc modification of OGT was observed in cells transfected with PHB mutants in comparison to wild type PHB in response to insulin only (Fig. 3B).
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