Sentence examples for mutant in vitro from inspiring English sources

Waz aptamer was transcribed by run-off transcription reaction (overnight at 37 °C) using a recombinant mutant form of T7 RNA polymerase (Y639F mutant), in vitro transcription buffer (50 mM Tris HCl, pH 7.5, 15 mM MgCl2, 5 mM DTT, 4% w/v PEG4000, and 2 mM spermidine), and 2 mM of each ATP, GTP, 2′-fluoro-modified CTP, and 2′-fluoro-modified UTP (TriLink Biotechnologies, San Diego, CA, USA).

The can2Delta mutant in vitro growth defect is largely attributable to defective fatty acid synthesis.

We also examined the effect of the cat-2 mutant in vitro.

The nature of this binding was studied using extracts of a microcystin-deficient mutant in vitro.

Recombinant PknA13 273 could phosphorylate recombinant wild type His-FtsZMTB but not the T343A mutant in vitro (Fig. 7A).

The ex vivo mutation data are consistent with the reduced activity observed in the K216A mutant in vitro.

Having established that a W32S substitution can significantly decrease the aggregation propensity of SOD1 mutants in vitro and in cells, we were curious if small compounds that have previously been shown to interact with W32 could achieve similar results.

Even though a Y. enterocolitica strain WA-C pTTSS), WA-C pTTSSslocated only LLO (but not Yops), induced superior MHC class I-restricted antigen presentation in DC compared to the ΔyopP mutants in vitro, this strain whichotranslocatedgnificantly conlyize mouse tissue or to induce CD8 T ceLLOresponses in vivo.

(H) Deadenylase assay of CNOT7 with wild-type TobN138, K63A and D95A mutants in vitro.

Compared to the traditional antibiotics, cationic antimicrobial peptides (AMPs) exhibit several unique characteristics, including the ability to rapidly kill target cells, broad spectrum activity against serious antibiotic-resistant pathogens in the clinic, and the relative difficulty in selecting resistant mutants in vitro (Jenssen et al., 2006; Huang et al., 2010b).

We also examine the H2O2 scavenging abilities of four pure scleractinian FPs and their colorless mutants in vitro.