CKI-ε (WT and KD mutant) constructs were provided by Dr. Xiaofan Wang (Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, USA).
Cell culture medium samples from NSP3 (Set III) and NSP4 (Set II) mutant constructs were collected at 12 h post-induction for evaluating the productivity of secreted ATH35L.
The mutant constructs were also engineered to express the β-lactamase CTX-M-15 as a tool to enable assessment of the activity of avibactam.
This wt chimeric construct showed impressive (>80%) protection against, virus challenge, on the other hand, the selectively disabled mutant constructs were less effective.
Deletion and site-specific mutant constructs were generated to identify the active regions in GST/III1H,8 10 and reduce construct size.
Mutant constructs were prepared by inverse PCR [80] with appropriate oligonucleotide pairs.
To determine which amino acid substitution dictates the fusogenic change, three mutant constructs were made.
These mutant constructs were used for transient transfection to Sf-9 cultured cells, a cell line from lepidopteran Spodoptera frugiperda.
The various mutant constructs were transfected into U937 cells and the expression of luciferase reporter gene was assayed.
Two mutant constructs were generated with PCR by reverse primers, huPTEN-miR144MulI-mut and huRB1-miR36MulI-mut.
The vector alone wt and mutant constructs were transfected into the CHO-DG44, NIH3T3, RK3 and in MCF10A cells.
