Sentence examples for mutant constructs was from inspiring English sources

Each of these mutant constructs was analysed individually.

Each of these mutant constructs was faithfully expressed in the 293-cell transient expression assay (see Fig. S2).

The ubiquitin-specific protease activity of USP25m and of all the mutant constructs was analyzed as described elsewhere [17].

Each of these mutant constructs was initially tested for faithful mutant protein synthesis after transient expression in 293 cells as shown in Figure 1B.

Later GSK3ß full length and mutant constructs was transferred to mammalian pcDNA3.1 expression vector TOPO cloning system and transformed to JM-109 compentent E.Coli strain following heat shock procedure.

To this end, 592 T>C and 1100G>A mutations were introduced to −1802CIP2ALuc by site-specific mutagenesis and the activity of mutant constructs was compared to wild type 1802CIP2ALuc in AGS cells.

CKI-ε (WT and KD mutant) constructs were provided by Dr. Xiaofan Wang (Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, USA).

Deletion and site-specific mutant constructs were generated to identify the active regions in GST/III1H,8 10 and reduce construct size.

Cell culture medium samples from NSP3 (Set III) and NSP4 (Set II) mutant constructs were collected at 12 h post-induction for evaluating the productivity of secreted ATH35L.

This wt chimeric construct showed impressive (>80%) protection against, virus challenge, on the other hand, the selectively disabled mutant constructs were less effective.

The mutant constructs were also engineered to express the β-lactamase CTX-M-15 as a tool to enable assessment of the activity of avibactam.