Sentence examples for variant constructs were from inspiring English sources

The three variant constructs were PCR amplified as BclI- AvrII fragments using specific primers (Table  2) and then cloned into the JMP62 URA3ex-YFP-N vector previously digested by BamHI and AvrII.

The FLAG HOT1 and the homeobox deletion variant construct were transfected using either Effectene (Qiagen) or Lipofectamine 2000 (Invitrogen) as the transfection reagent according to the manufacturers' instructions.

The luciferase activity of the p-198A variant construct was significantly lower than that of the WT p-198C promoter construct (p-198C/A ratio = 2, P = 0.006) (Figure 3B) suggesting that DC-SIGNR p-198A affects promoter activity.

When TRPA1 variant cDNA constructs were transfected into SH-SY5Y cells, the responses to TRPA1 agonists were smaller than those observed in transfected HEK293 cells, similar to our previous findings [ 12].

Where variant GFP-containing constructs were used, wells/dishes were transfected with 0.5 µg of each plasmid.

The transgenic PRE constructs of the Fab-7 and vg PRE were obtained by PCR amplification on genomic DNA using the primers shown in Table 2. PREs, variants and control constructs were cloned with NotI/ XbaI or NotI/ SpeI into the pKC27_ mw vector.

LOVpep variants, ePDZb1, and CRY2 variants used in bait constructs were fused at the C-terminus of Gal4BD in plasmid pDBTrp via homologous recombination.

For functional testing of the two common as well as the newly identified rare coding variants of TRAF3IP2 [ 2], constructs were generated by site-directed mutagenesis by using the QuickChange site-directed mutagenesis kit (Stratagene).

All of the variants thus constructed were found to give higher β-galactosidase activities than the control protein with the wild-type MifM sequence.

We show that antibodies raised against these PspC constructs are variant specific and prevent association between PspC and the complement pathway mediator, human factor H. We found that PspC variants differ in their capacity to bind factor H, suggesting that sequence variation within pspC reflects differences in biological function.

HT1080 human fibrosarcoma cells were transiently transfected with FUS-DDIT3-GFP variant constructs and FLT1 expression was measured by quantitative real-time PCR.