Sentence examples for mutant constructs resulted from inspiring English sources

Each of these mutant constructs resulted in faithful mutant protein expression as shown in Figure 3B.

Cells transfected with S80N/H94Y, Q249E and W802* c-CBL mutant constructs resulted in increased number of viable cells that was 132.3%, 120.8% and 147.9% higher respectively, relative to the empty vector control transfected cells and significantly different from the wild-type construct (p = 0.022, p = 0.049, and p = 0.008, respectively) (Figure 3B).

In MCF-7 cells all three mutant constructs resulted in greater promoter activity compared to the wild-type L6 construct.

After 24 h, the DHODH mRNA levels were equal for the wild-type and the three mutant constructs (results not shown), suggesting that the G152R and R346W mutant proteins showed reduced protein expression or increased protein turnover.

On the other hand, addition of ABA into the protoplasts transfected with the mutant mW60 GUS construct resulted in less than 1.5-fold induction of the reporter gene expression compared with the non-induced condition.

Overall, the improved mutant constructs with resulting high cleavage efficiency followed the previously described "cleavage pattern" [ 27] and Minskaia et al., manuscript in preparation, where stepwise amino acid deletions from the N-terminus of F2A resulted in linear increase in accumulation of uncleaved polyprotein and a decrease in the amounts of cleaved products.

Whereas the wild-type ProGL2 EYFP GL2 construct rescued all three mutant phenotypes regarding leaves, roots and seeds, the ProGL2 EYFP gl2ΔSTART construct resulted in null phenotypes indistinguishable from the gl2 loss-of-function mutant.

Details of individual mutant constructs and experimental results are presented in Figures S5, S6, S7 and S8.

On the contrary, introduction of the - AAK A- substitution in the F2A20 construct resulted in an increase in cleavage efficiency comparable to that of the mutant construct pGFP-F2A20mut-CherryFP with the - SGSR GAC- substitution.

Compared to wild type and Walker A mutant constructs, where the presence of ATPγS resulted in an approximate threefold drop in affinity, ATPγS binding by the ΔCARDs construct lowered the affinity about 1.4-fold.

These differences in mutant constructs may potentially explain discrepancies in our results.