Sentence examples for mutant constructs described from inspiring English sources

To map the site of interaction we used the Rinl mutant constructs described in Fig. 1A and found that the N-terminal region is dispensable for Rinl/Rab5a interaction.

For this assay, we prepared stable cell lines expressing each of the mutant HCF-1N constructs described in Figure 3 in an siRNA-resistant [6] and epitope-tagged form.

The generation of pCMV-myc-HSJ1 wild-type and mutant constructs was described previously (35, 54).

We tested if the ATPase function and the putative CUE motif are involved in this phenotype by overexpressing wildtype and mutant Fun30 using the constructs described above (Figure 9B).

The rad18 mutants used were generated in our laboratory using constructs described by Takeda and co-workers [28].

The pSG5- and pcDNA3-based rC/EBPα wt and mutant constructs have been described earlier (Calkhoven et al, 2000).

The transcriptomes of rpoE and mclA deletion mutants (constructed as described in Materials and Methods) were compared with the wild-type H. influenzae using RNA-seq.

The pEGFP-C1-SENP3 and its mutant constructs have been previously described (Yan et al., 2010).

The ubiquitin-specific protease activity of USP25m and of all the mutant constructs was analyzed as described elsewhere [17].

PIP2 hydrolytic activity of PLCζWT, PLCδ1WT and mutant constructs was assayed as described previously [ 15].

The Lrp4ΔICD/ΔICD knockin mice were generated using a replacement vector based on the construct described for the LRP4 ECD mutant.