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Exact(6)

For each competition experiment we compute the relative fitness Wm of the mutant, by comparing the fold expansion of the mutant and wild type [33], [83].

To understand the basis for the low NPQ in the pgr7 mutant, we sought to determine whether any previously identified NPQ factors were affected in the pgr7 mutant by comparing pgr7 and Ler plants grown on soil.

I constructed various L1 mutants shown in Figure 1A and determined the ubiquitination levels for each mutant by comparing the mutant with wild-type L1 (L1WT, Fig. 1B).

We next proved that the an- t1 mutant is really an angustifolia null mutant, by comparing its phenotype to previously well characterized angustifolia null mutants.

Although it is difficult to clone the Fhb1 by map-based cloning due to the deletion of a large chromosome fragment in the mutant, by comparing gene expression patterns between Wangshuibai and NAUH117 during Fg infection based on transcriptome analysis, it is feasible to uncover the mechanisms underlying FHB resistance, especially mediated by Fhb1.

To assess the pressure threshold for activation of the MscS channels, a pressure ratio (PL PS) was determined for each mutant by comparing the pressure required to gate MscL with that required to open the MscS channels in the same patch, as employed previously (13).

Similar(54)

We then analyzed which of these unstable mRNAs are stabilized in embryos from aub or spn-E mutant mothers (aub or spn-E mutant embryos) by comparing mutant and wild-type embryonic transcriptomes.

Of these mutants, we succeeded in classifying srs3, Srs5, d1, and d61 into cell elongation- or division-type mutants by comparing the cell length and cell number of the inner-epidermal cells of lemma (Izawa et al. 2010; Kitagawa et al. 2010; Segami et al. 2012).

Therefore, we investigated the possibility of an unbalanced expression of the wild-type (wt) and the mutant alleles, by comparing the sequence of PRPS1 mRNA at the mutant position, amplified by RT-PCR from PBMC RNA, with that obtained from genomic DNA (Supplementary Methods).

Another set of experiments was conducted to directly assess the effects of STARs within the mutant strains by comparing the levels of silencing in STAR-less (URA3-tel and GF6ΔSTAR) and STAR containing (GF2 and GF6) constructs.

We therefore further extended our quantification methodology to help address any small changes in nuclear Armadillo accumulation that might have occurred upon mutant overexpression, by comparing levels within the expression domain with those in wild type neighbours.

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