Sentence examples for mutant by determining from inspiring English sources

We scored the number of infected cells in the chamber infected with the gD mutant by determining the total area of infected monolayer.

We further screened these 86 mutants by determining their lignocellulase secretion capacity through batch culturing with microcellulose (2% [w/v] Avicel) as the carbon source and yeast extract (0.75% w/v) as the nitrogen source.

The quantitative influence of the different scaffoldin modules of CipA to cellulose consumption was deduced from fermentation assays of the mutants by determining the maximum hydrolysis rate (V max ) and t max, which is the cultivation time at V max [ 17].

Given their drastic biochemical deviations, we also validated the integrity of the S150A and T151A mutants by determining their crystal structures, which illustrated that the mutations did not have any adverse effects on the overall fold of the protein or on the G5 loop.

The relative activity of the mutants was obtained by determining the average slope of the aggregation curves obtained for the mutants and interpolating this number back into the standard curve to calculate the ratio of wt Spy: aldolase that would give with an activity comparable to the mutant.

The binding affinities of WT and Lon mutants were evaluated by determining the concentration of λ N (KλN) needed to achieve 50% maximal ATPase stimulation.

The lacZ mutant frequencies were then calculated by determining the ratio of mutant pfu to total pfu.

We also analyzed the mechanism whereby the present mutation caused the generation of two different mutant transcripts in more detail by determining the order of intron removal.

In this study, the thermodynamic stability of the oxidized and reduced L-SH2 domains, as well as that of the mutants at equilibrium, was characterized by determining the free energy of unfolding.

Nonetheless, much lower activity than PKM2 WT was detected with this mutant as determined by pyruvate kinase assay.

The sequence of the disrupted ORF for each attenuated mutant was determined by either cloning or PCR-amplifying and sequencing the flanking regions.