Sentence examples for mutant by fusing from inspiring English sources
To further verify the repressive role of vosA in conidiation, we generated the vosA overexpression mutant by fusing the vosA ORF with the inducible alcA promoter [25].
The ΔgerP-null mutant was isolated by fusing the first 10 codons of gerPA to the last 10 codons of gerPF, with stop codons and restriction sites inserted in between, as described above.
Meanwhile, deletion ESE-1 binding site mutant promoters were formed by fusing polymerase chain reaction (PCR) based on the PGL3-basic firefly luciferase reporter vector with GP73 promoter (−2618/−19) [ 32].
A FcR-binding deficient mutant of PDL241 (241-G2M3) was made by fusing the PDL241 variable domains to human IgG2M3 Fc domains [ 20].
ChIP experiments revealed that Hos2 peaks at the ste11 promoter and that its recruitment was strongly dependent upon the S5-phosphorylated CTD as its occupancy was decreased in a CTD S5A mutant, which is kept viable by fusing the human capping enzyme Mce1 (Schwer and Shuman, 2011).
Generation of transmitochondrial cybrids with 100% mutant load can overcome this difficulty; these are created by fusing enucleated cells containing mutant mtDNA with a 'ρ zero' nuclear donor cell line, which has been depleted of mtDNA (King and Attardi, 1989) and then selecting for cybrids with 100% mutated mtDNA.
Firstly, cloned HBB −28 (A>G) mutant homozygous human embryos were generated by fusing skin fibroblast cell from the patient with in vitro matured oocytes.
Cloned HBB −28 (A>G) mutant homozygous human embryos were generated by fusing lymphocyte cell, from peripheral blood of the patient, with in vitro matured oocytes.
Here, we tested this idea by fusing TGFαL3 to SEA (D227A), a mutant of SEA defective for MHC-II [ 23].
Mutations in NES-A that specifically impair NMD by blocking Upf3p export were identified by fusing a bona fide Crm1p-binding NES to the mutant Upf3p proteins and then selecting for those that regain function in NMD.
He produced a giant larva by fusing two embryos.
