Sentence examples for muscles were processed from inspiring English sources

The frozen fish muscles were processed to obtain their extracts in ice-cold saline as described by Anwar et al. (2004).

Hind-limb muscles were processed as previously described (Mitchell et al, 2010).

From each heart, two midventricular slices (at the level of the papillary muscles) were processed and embedded in paraffin, because they are most representative of the scale of LV remodeling and the extent of the transmural scar [ 27].

Serial cryosections of human ocular MR muscles were processed for in situ hybridization with probes, specific to mRNA transcripts of 1 or ß-slow, 2a, 2x, 2b, embryonic and neonatal MyHC genes [ 44].

The tibialis anterior muscle was processed for mitochondrial isolation (Lenaers et al. 2010) and soleus and EDL muscles were processed for electron microscopy or rapidly frozen in melting isopentane (Fluka, Zwijndrecht, The Netherlands), and stored at −80°C.

The muscles were fixed in 4% PFA in PBS for 15 minutes (mid-late-symptomatic animals) or 10 minutes (early-symptomatic animals), and all muscles were processed for immunohistochemistry.

For fiber type analysis, serial sections of the plantaris muscle were processed either with a set of antibodies against the various myosin heavy chain (MyHC) isoforms, as previously described [19] or stained for the presence of SDH enzyme activity.

Cryosections of skeletal muscle were processed for immunofluorescence using a previously described protocol [ 11] with the above antibodies to respiratory chain enzyme complex proteins (green fluorescence); antibody to porin (Abcam ab14734; red fluorescence) served as reference control for mitochondrial density and distribution.

For the estimation of total protein by the method of Lowry et al. (1951), 0.5 g of a frozen muscle was processed as described before but homogenized in 4 ml ice-cold 0.5 N NaOH.

The masseter muscle was processed for histological analysis, conventional and in situ zymography, and immunohistochemistry.

Frozen muscle was processed by a method similar to that described by Hartikka and coworkers [ 20].