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EGFP signals of the dissected skeletal muscles were detected by an animal imaging system.
During the training phase, 3.5 s epochs corresponding to single characters were selected using an algorithm in which bursts in compound EMG (the sum of rectified EMGs from all muscles) were detected which crossed a threshold (0.5 standard deviation from the intertrial level) designated the epoch onset.
On some slides, in which both the pectoralis major and minor muscles were detected, we evaluated all lymph nodes without any information on pathological findings.
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The concentration of 18 cytokines and chemokines in the damaged muscles was detected using MILLIPLEX MAP Multiplex Assay Kits, according to the manufacturer's instructions (Millipore).
Concomitant activity of QF and hamstring muscles was detected also during push-off phase (30 50% of gait cycle), showing a large variability intra and inter subjects and a lower occurrence frequency (around 25% of strides).
The presence of human BMP-2 or BMP-7 mRNAs transcribed from pCAGGS-BMP-2 or pCAGGS-BMP-7 in the electroporated muscles was detected 1,3,5,7 and 9 days after treatment (Fig. 3).
No differences in the transcript levels of Foxo1 and Foxo3a in GAS muscle were detected between wild type and ob/ob mice, but a tendency towards a down-regulation of both transcription factors was found after leptin treatment (Figure S3).
Antibodies to nuclei and smooth muscle were detected at titers of 1 40 and 1 160, respectively.
In addition, a number of miR previously reported to be expressed in mammalian muscle were detected, having a variety of abundance patterns through muscle development.
Suggestive genomic regions affecting glycogen content in the liver and in the muscle were detected on chromosomes 18 and 9, respectively (Table 3).
Histological (ragged red fibres, cytochrome c oxidase fibres), biochemical (respiratory chain defect) and genetic abnormalities (multiple mitochondrial DNA deletions, mitochondrial DNA depletion) in skeletal muscle were detected more frequently in older age (Tables 3 and 4).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com