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Brains were processed for Golgi staining, and hippocampal CA3 dendritic architecture was quantified.
All brains were processed together for CB1 receptor binding using 3H CP55,940 in prefrontal cortex, striatum, amygdala and hippocampus.
Brains were processed using either a manual dounce based approach or enzymatic digestion using type IV collagenase.
Rats were perfused and their brains were processed for Fos immunohistochemistry 90 min after the i.v. treatment.
or vehicle (DMSO, 1ml/kg, i.p .. Their brains were processed for the detection of c-Fos protein, used as an indicator of brain activation.
Cognitive performance was assessed in the Morris water maze (MWM) between 11 and 15 days post-injury and the brains were processed for synaptophysin immunohistochemistry and routine electron microscopy.
After 6 and 12 weeks of implantation, sections of brains were processed with Nissl and Glees staining for revealing neural cell bodies and fibers, with DAB histochemistry for detecting the blood vessels, as well as with immunocytochemistry for recognizing GFAP.
All subjects (n=10) were sacrificed 28 days after the last BrdU injection (50 mg/kg X 3 days) and their brains were processed for immunohistochemistry. Results illustrate a decrease in the number of BrdU-positive cells as well as different distribution of these cells in the dentate gyrus of irradiated animals.
The brains were processed as described above.
Fresh frozen brains were processed as described [53].
PND0 brains were processed as described for embryonic brains.
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