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The muscles were placed in a Petri dish, and myofibers were isolated under a microscope.
The muscles were placed into their original position and the skin incision was closed using a 6 0 nylon suture.
For fixation, muscles were placed in 4% PFA for 1 h, 100 mM glycine for h, then 30% sucrose overnight at 4°C.
Muscles were then placed in the same solution containing 0.2% collagenase type I and 10% FCS and incubated with 5% CO2 in air at 37°C for 90 min. Muscles were placed in culture medium (DMEM and 10% FCS) for 30 min and then single fibers were dissociated by gently triturating with a plastic Pasteur pipette.
Briefly, harvested skeletal muscles were placed in 5-ml Falcon tubes each containing 1 ml of ice-cold Protein Lysis Buffer (Cell Signaling Technology, Danvers, MA) supplemented with 50 mM dithiothreitol (DDT) and 1 mM phenylmethylsulphonyl fluoride (PMSF) and homogenized with an Ultra Turrax T25 Polytron™ homogenizer.
In brief, isolated muscles were placed directly into enzyme solution, without any additional mechanical disruption with scissors.
Similar(46)
Contractures are avoided if muscles are placed under tension for as many hours as possible but stretching exercises do not necessarily result in prevention of contractures unless maintained for at least six hours a day [16].
This will help you understand where the muscles are placed so you can draw an anatomically correct bear.
The opposite Achilles tendon and soleus muscle were placed in RNAlater for subsequent analysis of gene expression of Collagen type I (COL1A1) and Collagen type III (COL 3A1).
The microdialysis catheters in liver and muscle were placed using a splitable needle (M Dialysis AB, Stockholm, Sweden) as previously described [ 19].
Approximately 200 mg of gastrocnemius muscle were placed in glass homogenization tubes containing 4 mL of homogenizing buffer (50 mM phosphate buffer, 1 mM Ethylenediamine tetraacetic acid, pH 7.4).
More suggestions(15)
brains were placed
muscles were injected
muscles were used
muscles were implanted
muscles were examined
muscles were compared
muscles were spared
muscles were analysed
muscles were mounted
muscles were dissected
muscles were frayed
muscles were cramped
muscles were detached
muscles were prepared
muscles were incubated
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