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The experimental approach involved multiplex measurement of cytokines, chemokines and growth factors produced by activated monocytes, as well as microarray profiling.
Multiplex measurement of a subset of the differentiating biomarkers provided high sensitivity and specificity in the diagnostic discrimination of RA.
To investigate circulating miRNAs, a highly versatile multiplex detection platform to support the multiplex measurement of circulating miRNAs is being developed.
As for SYBR Green method, it can be used for the cases when the multiplex measurement of heteroplasmy level of some mutations would be impossible.
In this initial study, multiplex measurement of a subset of the differentiating biomarkers provided high sensitivity and specificity in the diagnostic discrimination of RA: Use of 3 biomarkers yielded a sensitivity of 84.2% and a specificity of 93.8%, and use of 4 biomarkers a sensitivity of 59.2% and a specificity of 96.3%.
Spanning the middle ground between low-throughput approaches like qPCR and high-throughput approaches like microarrays and RNA sequencing, the Nanostring™ nCounter® platform is a middle-throughput approach based on direct multiplex measurement of gene expression, effectively 'counting' transcripts using barcoded probes and automated quantification of single molecule digital imaging [ 14].
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Beyond the details of our particular experimental model, the achievement of reliably quantitative multiplex measurements of gene expression in single cells is meaningful for a wide variety of applications.
Quantitative mass spectrometry targeting specific molecules rather than random sampling of the whole proteome, e.g., multiple reaction monitoring (MRM), offers an efficient and effective means to multiplex the measurement of several candidates in patient samples, thereby omitting the need for antibodies prior to clinical assay design.
The use of such multiplex measurements will itself generate significant problems for method validation, such as crossreactivity, interference, sensitivity and stability.
First, we cannot exclude that multiplex measurements did not correlate with single assays for some of the circulating factors.
Such panels may represent a series of single measurements or, more likely, as modern technological platforms emerge, by multiplex measurements.
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