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STRT uses a template-switching mechanism to simultaneously introduce a molecular barcode and an upstream primer-binding sequence during reverse transcription, which permits multiplex sequencing of multiple cells simultaneously.
Multiplex sequencing of 16S rRNA tags revealed that both types of RS induced several significant compositional alterations in the fecal microbial populations, with differential effects on community structure.
The bar-coded multiplex sequencing of RILs and the construction of high density bin map were as described by Xie et al [15].
Recently, the ecological study of the human gastrointestinal microbiota has gained enormous momentum through the development of high throughput multiplex sequencing of 16S rRNA tags [24], [25].
Multiplex sequencing of 101 bp paired-end reads was performed on an Illumina HiSeq2500 instrument.
Multiplex sequencing of transcript 3′ ends identifies differential transcript abundance independent of gene annotation.
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Equal volumes of libraries were pooled and subjected to multiplex sequencing on the MiSeq sequencer (Illumina).
Representative purified long-range PCR amplicons from the four loci of one sample are shown in Additional file 5. We used a novel method, MIT-NGS that combined long-range PCR and Illumina MiSeq-based multiplex sequencing for HLA typing of four loci of 96 individuals in a single run.
Library 3 was a multiplex sequencing library of three equimolar indexed samples, which was enriched using one target enrichment reaction.
Library 4 was a multiplex sequencing library of nine equimolar indexed samples, which was enriched using one target enrichment reaction.
A library-specific barcode for multiplex sequencing was part of a 3′-sequencing adapter.
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