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Biochip array technology allows multiplex determination of multiple biomarkers from a single sample [ 16, 17].
For this purpose, a biochip array was designed and developed for the multiplex determination of nine serum markers allowing for low inter-analysis variability, decreased workload and faster processing time as well as lower costs due to high-throughput automation.
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Additionally, DNA preparations derived from field samples were used to evaluate the utility of the single and multiplex PCRs for determination of infection status.
Thus, the results demonstrated that the nanobiochip sensor served as a specific and multiplex method for the determination of multiple salivary biomarkers from saliva and serum with sensitive signals and low DL within a short time [ 161].
Representative isolates, one per PFGE type, were further characterized by MLST [ 5], and by a multiplex PCR for the determination of the presence of Panton-Valentine leukocidin (PVL) genes (lukS-PV – lukF-PV) [ 36], SCC mec type I-IV [ 4], a PCR for the detection of ccr-gene and mec-gene complex [ 37], and by PCR for the detection of ccrC of type V SCC mec [ 7] as described earlier.
For a given isolate, integration of PCR and multiplex pyrosequencing results allows the determination of the ESBL-producing status (available at https://ucl-irec-ctma.shinyapps.io/Pyrosequencing-TEM-SHV/).
The findings reported here are proof-of-principal for value of sequential multiplex PCR capsular serotyping for determination of regional serotype distribution and specifically among cases of pneumococcal meningitis.
Multiplex PCR method was used for determination of GSTM1 and GSTT1 polymorphic deletions.
We developed an accurate multiplex quantitative real-time PCR for synchronized determination of mtDNA and nuclear DNA (nDNA).
After collecting the samples, the standard diagnostic method for detection of bacteria were performed and after extracting the DNA, the multiplex PCR method has been used for determination of Salmonella serotypes.
A common design problem involves the determination of a wavelength-division multiplexing (WDM) system.
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