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Although the multiplex diagnosis of three viral diseases above was demonstrated as proof-of-concept here, the proposed LFA system can be applied to multiplex point of care diagnosis of other intractable diseases.
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This work revealed the comprehensive evaluation of sample processing prior to the multiplex PCR diagnosis of C. difficile directly from clinical stool samples.
Patients were identified as multiplex if the diagnosis of SLE could be documented in one or more family members.
This DNA was used for the respective molecular assays, namely; 1) semi-nested multiplex PCR for diagnosis of malaria and confirmation of the Plasmodium species 13; and 2) PCR analysis of P. falciparum microsatellite loci.
A statistical model, designed to evaluate the accuracy of this multiplex biomarker approach for diagnosis of TCC, predicted the presence of disease with 90% accuracy.
SeptiFast (Roche, Germany) remains the most thoroughly examined PCR multiplex platform for rapid diagnosis of BSI.
These advances have resulted in the appearance of numerous publications regarding the application of multiplex PCR in the diagnosis of human brucellosis.
It is possible that by adding other relevant species to a multiplex PCR the molecular diagnosis of BV can be even more refined compared to our BV-PCR assay.
We used MPB64 gene primers for the detection of MTB genome that was a part of previously validated multiplex PCR assay for the diagnosis of posterior uveitis [7].
About 2 years ago, multi-target PCR was shown to have high specificity (100%) and sensitivity (73.68%) in a series of 19 patients with suspected ocular TB (Sharma K et al., Novel multiplex polymerase chain reaction for diagnosis of intraocular tuberculosis. Poster at: AAO Annual Meeting, Orlando, 2011).
We evaluated a multiplex RE-PCR for the diagnosis of key Trichostrongylus spp. Genomic DNA amplification of Trichostrongylus colubriformis, Trichostrongylus axei and Trichostrongylus vitrinus was achieved as standard sample using specific primers located in the second internal transcribed spacer (ITSII) of nuclear ribosomal DNA (rDNA).
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