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This multiplex capability of the platform makes it a good tool for complex phenotyping in large scale toxicology.
To demonstrate the multiplex capability of the cell chip, we printed chips with both A549 non-small-cell lung cancer cells and HeLa cervical cancer cells.
However, EDC fixation may limit the multiplex capability of miRNA and protein co-detection because EDC cross-links can cause epitope masking [ 79].
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We predict that this method is adaptable for monitoring other host guest systems with various types of NPs, with the inherent multiplex capabilities of the mass barcode approach facilitating high-throughput screening.
Owing to multiple access interference cancellation ability of MIMO, bi-directional flow multiplexing capability of network coding in combination with an efficient channel access scheme of TDMA/TDD, MIMO two-way relay provides significantly high end-to-end capacity.
It is also difficult to compare tests because of the differing "value" of a technology (e.g., multiplex capability, appropriateness of the site to conduct the screening service).
Alban et al [ 24] used an Escherichia coli lysate "spiked" with varying amounts of four different known proteins to test a novel experimental design that exploits the sample multiplexing capabilities of DIGE by including a standard sample in each gel.
In summary, i-PITT offers i) superior targeting efficiency, ii) multiplexing capability to insert different cassettes simultaneously, iii) reduction in the number of animals used; an important animal welfare consideration, iv) uniform genetic background.
The assay combines the multiplex capabilities and specificity of ligation reactions with high-throughput real-time PCR in the OpenArray™, resulting in a flexible, quantitative multiplex diagnostic system.
As a sensitive and specific assay with multiplex capabilities, RNAscope allows quantitative measurements of RNA levels in the context of the tissue [ 11] as well as reliable detection of low abundance RNA molecules by flow cytometry [ 16].
Because of the public health importance of technologies with multiplex capabilities, this criterion was allotted two hundred points.
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