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In addition, we developed a multiplex assay that simultaneously amplifies four fragments in a single PCR reaction.
Real-time PCR assays for nucleic acid testing (NAT) of hepatitis viruses A-E and for HIV-1 and HIV-2 have been developed; however, a multiplex assay that can simultaneously detect all of these agents is not yet available.
Alternatively, a single multiplex assay that includes both TBP and FTH1 loci may be useful.
A multiplex assay that can detect contamination of other cell lines within a mixed sample would be useful.
Our real-time RT-PCR is a multiplex assay that employs a mixture of 2 sets of inhouse designed primers and dual-labeled fluorescent probes that specifically target 2 different regions of the HA gene of H5N1.
A multiplex assay that encompasses the citrullinated antigens that are actually present in RA joints has the potential to improve the diagnosis of RA by enabling more comprehensive detection of ACPA responses as well as stratification of RA on the basis of the fine specificity of ACPA responses.
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We developed two multiplex assays that simultaneously screen 22 nucleotides in the KRAS, NRAS, BRAF and PIK3CA genes for mutations.
For this reason, we developed two multiplex assays that allow the genotyping of SNPs at these two genomic regions which have been shown to be associated with variable HbF levels in different populations.
This property can be exploited in iLAMP method for designing multiplex assays that detect different protein targets simultaneously.
As of 2010, assays that incorporate an array of antibodies against specific protein marker molecules are an emerging technology; there are hopes for these multiplex assays that could measure many markers at once.
Further studies should be done to generate multiplex assays that detect all common epidemic respiratory adenoviruses.
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