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In the present study, we investigated the kinetics of 24 cytokines in rats developing AIA using a multiplex immunoassay that allowed a highly sensitive biological follow-up of multiple mediators from a limited amount of biological sample [ 15].
Since insulin regulates FOXO1A transcriptional activity predominately through AKT [9], the effect of overexpression of screen hits on insulin-induced AKT phosphorylation was investigated at six time points over a 24 hour period using a high throughput multiplex immunoassay that detects total AKT and phosphoAKT (S473) proteins simultaneously.
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The levels of sE-selectin, sICAM-1, sVCAM-1, MMP-9, vascular endothelial growth factor (VEGF), granulocyte-colony stimulating factor (G-CSF), and granulocyte macrophage-colony stimulating factor (GM-CSF) were determined using a multiplex sandwich immunoassay that was performed using a Luminex 200 (Luminex; Austin, USA) and xMAP technology [ 19, 20].
The Magpix is a multiplex immunoassay system that uses magnetic bead technology, with LED optics and charge-couple base device (CCD) camera to illuminate and detect differentially fluorescent dyed beads.
In this study, we developed a multiplex immunoassay system that combines the suspension and planar microarray formats within a single layer of polydimethylsiloxane (PDMS) using soft lithography technology.
Simple Plex is a microfluidic multiplex immunoassay device that offers miniaturized, and automated analysis of protein biomarkers.
The use of multiplex immunoassays entails research that is basically hypothesis-generating.
Here, we developed a multiplex immunoassay platform within microfluidic devices that combines suspension and the planar microarray format.
= no accession # Principal component analysis applied to the multiplex immunoassay (RBM) data set revealed that each of the subjects could be identified based on levels of 89 plasma proteins.
One of the most widely used approaches for quantitative multiplexing of proteins is multiplex immunoassay [ 8].
De Jager et al. [3] have described methods to avoid heterophilic antibody interference in plasma and synovial fluid that improved the performance of the multiplex immunoassay.
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