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A gene expression study was performed using a multiplex platform that reduced errors due to sample processing, dilution, and amplification.
MassTag PCR is a multiplex platform that enables inexpensive, sensitive, and simultaneous detection of multiple pathogens (7 – 10 ).
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Particularly notable is the implementation of multiplex platforms that allow a single type of specimen preparation and simultaneous (or nearly simultaneous) screening for multiple different disorders.
A gap-LCR assay has also been combined with a fluorescence polystyrene microsphere measurement platform that allows multiplex analysis of SNP [24].
These studies, and those from other groups, have identified a set of proteins that might act as biomarkers relevant to AD. However such findings require replication, in large studies, ideally using samples drawn from more than one cohort source and using a platform that enables multiplexing.
We developed a high-throughput method for preparing paired-end sequencing libraries for the Illumina platform that allows for multiplexing up to 96 individual DNA samples without the use of expensive sample preparation kits or specialized DNA fragmentation equipment, and with starting amounts of DNA as little as 100 ng per sample.
We measured different mediators of inflammation with the Luminex multiplex platform and found that apremilast treatment did not significantly change the levels of IL-1α, IL-10 and IL-6 in the air pouch exudates (Fig. 2c).
Based on these encouraging preliminary data, we believe that the Q-Plex technology is a viable platform that can be exploited as an efficient, highly accurate tool to quantitate multiplex panels of diagnostic proteins in biologic specimens.
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SNP data can be obtained using one of the numerous uniplex or multiplex SNP genotyping platforms that combine a variety of chemistries, detection methods, and reaction formats.
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