Sentence examples for multiplex protocol that from inspiring English sources

Ponderosae (Fig. 1; Appendix 2) using a PCR multiplex protocol that integrates fluorescent labels during PCR (Culley et al., 2008; Culley et al., 2013).

A multiplex PCR protocol that amplified the consensus portion of the B subunit of the cytolethal distending toxin gene (cdtB) as described by Toth (16 ) was modified by using each of the primer pairs (s1/as1 and s2/as2) individually to screen for cdtB in all isolates.

Since concentration and size distribution (as assessed by ethidium bromide agarose gel electrophoresis) of genomic DNA isolated from FFPE tissue are inadequate predictors per se for aCGH success, we have developed a method for DNA isolation from FFPE tissue with a subsequent simple and reliable multiplex PCR protocol that predicted successful aCGH with high accuracy.

To address these limitations, we designed a multiplex real-time PCR protocol that allows amplification of control and target genes in the same reaction and precise size determination of bcl-2/JH fusion sequences by capillary electrophoresis.

Moreover, the detection limits of the multiplex real-time RT-PCR assay were directly compared to a widely used real-time RT-PCR assay protocol that is recommended by the WHO.

The authors reported that their multiplex protocol costs €1.55/genotype (without including reagent cost); if additional mutations in the same amplicon (four mutations have been observed in Greeks) can be detected, the cost per genotype could be reduced to €0.66/genotype.

Genotyping was performed using a multiplex protocol (see Supporting Information).

Microsatellite genotyping was conducted using a published multiplex protocol [ 39].

A multiplex protocol was used for L10, CG49 and CG108 (Taris et al. 2005).

Nevertheless, the approach requires complex fabrication protocols that limit throughput and multiplexing capabilities.

Although a multiplex PCR for detection of B. ovis, A. seminis, and H. somni has been previously described [ 8] that protocol allows only for identification of Brucella spp. at the genus level, whereas here we describe a multiplex PCR that is the first species-specific assay.