Sentence examples for multiple expression vectors from inspiring English sources

Although we successfully cloned all three selected F. tularensis genes and three of the four selected human genes into the LUMIER expression vectors and transfected them into the human HEK293T cell line, we were essentially unable to express the bacterial proteins using multiple expression vectors.

Initially, native MVP (MVP-n) cloned from the genome of M. jannaschii was tested for expression in multiple expression vectors with disappointing results (Table 1).

We used a high-throughput cloning approach using the Gateway system and liquid-handling robots for cloning the 22 ORFs into multiple expression vectors, expression screening, and activity screening.

Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order to analyze the large number of cDNAs available in the post-genomic era.

Several other Pseudoalteromonas(28, 29) and NRPS biosynthetic pathways have been heterologously expressed in E. coli previously under the control of a single non-native inducible promoter (T7, Plac_CTU, or PtetO), with larger pathways spread across multiple expression vectors.

The ability to pre-screen multiple expression vectors for secreted protein production on a small scale and thus identify failures prior to the larger scale transfections would reduce time, cost of reagents and allow an increased number of proteins to be produced at the large scale.

Multiple mammalian expression vectors, including plasmid and modified vaccinia virus Ankara (MVA) vectors expressing the HAs of two H5N1 influenza viruses and the HA of the recently emerging pandemic H1N1 (2009) virus, were developed.

In this work, two multiple baculovirus expression vectors (BEVs) with PRRS virus envelope proteins were engineered in order to generate PRRS VLPs: on the one hand, Gp5 and M cDNAs were cloned to generate the pBAC-Gp5M vector; on the other hand, Gp2, Gp3, Gp4 and E cDNAs have been cloned to generate the pBAC-Gp234E vector.

However, creating a suitable cloning plan becomes increasingly difficult in complex strategies requiring repeated insertions and or large recipient vectors such as in constructing multiple shRNA expression vectors for RNAi studies.

In contrast, the Tol2 system is a convenient method for integrating conventional and multiple plasmid expression vectors into the host cell genome to achieve stable expression.

The user can also add a variety of adaptor assemblies at the DNA sequence level to facilitate the virtual and physical cloning of the constructs into multiple defined expression vectors.