LOX family members are widely expressed in multiple tissues, and their expression sites are not completely consistent (10), suggesting that every member of the LOX family may have a unique function in different types of tissue.
In the case of multiple expression and functional analysis, proper restriction sites have to be selected according to the different sequences of genes in above-mentioned step I, and then step II is repeated.
Analysis of multiple expression data sets do not suggest that other tissues that contain abundant goblet cells, for example the intestines, are major sites of LPLUNC1 expression.
This may be due to a wide range of factors, including multiple polyadenylation sites, antisense expression, the sequence of the probes, position of the probe on the array [35] [37].
In addition, some have incorporated target site location, multiple target sites and expression profiling [ 1].
Overlaying a peer to peer workflow management system on multiple independent sites decouples task expression from resource allocation, i.e., the host resource managers allocate processing elements to host the peers, allowing the peers execute multiple workflow tasks.
Variation in a single patient's tumor profiling has been identified by groups measuring expression arrays from multiple tumor sites.
The ORF of CsLOX1 was cloned to multiple cloning sites of the expression vector pPIC9K, and a recombinant plasmid, namely pPIC9K/ lox1, was constructed.
To generate the constructs expressing AtSUMO1 GG) and AtSCE1a (pACYCDuet-AtSCE1-AtSUMO1), the AtSUMO1 and AtSCE1a coding sequence fragments were separately cloned into the 2 multiple cloning sites of the expression vector pACYCDuet-1 (Novagen, Germany).
A set of ten LIC-compatible vectors for in vitro and in vivo protein expression were constructed by inserting PCR-generated oligonucleotide fragments into the multiple cloning sites of commercially available expression vectors.
