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However, a synthetic version of MVP (MVP-s), in which 62 of the archaeal codons were altered to bacterial codons (see Experimental Procedures), was successfully expressed in several expression vectors and cell lines (Table 1).
Recombinant cathepsin L could not be expressed despite the use of several expression vectors and E. coli strains (results not shown).
Several expression vectors for different forms of surface display have been developed and, coupled with improved recombination strategies, represent progress towards a refined tool for use in functional genomics and in vitro protein evolution.
Transformation of several expression vectors was performed as described [68].
Since one of the goals of our lab is to express recombinant proteins (using plant viral vectors), we often need to clone specific genes of interest in several expression vectors [14].
The regions shown in Figure 4B were cloned upstream of a TATA box and cotransfected along with several expression vectors.
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In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein.
Several bacterial expression vectors have been constructed to produce modular proteins containing from the N- to the C-terminus: the FLAG epitope, a cleavage site for a protease, a PTD, human ubiquitin or SUMO-1, and either GFP or the HA epitope.
Several types of expression vectors have been used for recombinant protein expression in Chinese hamster ovary cells (CHO) which usually result in variable and unstable levels of expression.
Several Asd+ expression vectors with different origins of replication were transformed into E. ictaluri ΔasdA01.
To create a balanced-lethal system in E. ictaluri, several Asd+ expression vectors harboring the SD-asdA gene sequence from Salmonella Typhimurium UK-1 with different origins of replication, (Table 2) [19] were transformed into E. ictaluri ΔasdA01 to evaluate their complementation and stability.
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