The tricistronic reporter construct was developed by fusing pieces from numerous different expression vectors.
Numerous inhibitory RNA expression vectors have been described in recent years [ 25, 26, 28, 29, 36, 42- 51].
To maximise and optimise the production of recombinant products, recent molecular research has focused on numerous issues including the design of expression vectors, optimisation of gene copy number, co-expression of secretory proteins such as chaperones, engineering of glycosylation and secretory pathways, etc.
Numerous groups, including ours, have described various expression vectors in recent years [ 25- 28, 32, 33, 36, 41- 51].
There are numerous commercial and non-commercial E. coli expression vectors available that incorporate fusion tags (for both purification and enhanced solubility), Gateway or LIC technologies, protease cleavage sequences and regulable expression levels [ 7].
The application of minireplicons derived from broad-host-range plasmids RSF1010, pBBR1, RP4 and pSa for the construction of expression vectors and the use of numerous, versatile promoters extend the range of feasible expression levels considerably.
Numerous broad-host-range shuttle vectors, promoter-probe vectors, expression vectors and other special-purpose vectors are available now.
Numerous chemical and physical methods have been used to introduce DNA expression vectors into mammalian cells both in vitro and in vivo, including, but not limited to, calcium phosphate precipitation, microinjection, electroporation, receptor-mediated gene transfer, particle guns, viral vectors, polyfection and lipofection [ 1].
subtilis shuttle expression vectors.
Geminivirus-derived vectors can be classified into expression vectors and virus-induced gene silencing (VIGS) vectors.
