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Exact(9)
pFLuc TF reporter constructs encoded FLuc with a minimal TATA box from the herpes simplex virus with one or multiple enhancer elements upstream of the promoter that were specific for a particular TF (Panomics, Fremont, CA, USA).
Lastly, genes are frequently regulated by multiple enhancer elements or clusters of enhancer elements.
Further studies have identified multiple enhancer elements involved in this regulation [ 8- 11].
As multiple enhancer elements were identified in this region, it was suggested that they can regulate the transcription of Myc.
The enrichment of H3K9me2 additionally extends into domains that contain multiple enhancer elements, leading to their developmentally-linked inactivation.
In addition to loss-of-function alleles, gain-of-function mutants should also be discovered since the SK population was developed using a vector carrying multiple enhancer elements.
Similar(51)
Multiple enhancer-blocking elements are arrayed across HS2-6.
We tested expression constructs in which the minimal SV40 promoter was preceded by multiple 200-bp enhancer elements.
Although long considered an enhancer, our studies indicate that the regulatory region in the IL-2 promoter is better considered as a proximal regulatory element, since it lacks multiple properties associated with enhancer elements.
The strength of reporter gene expression increased with multiple enhancer cassettes, plateauing with the use of four enhancer elements (4 × Grm6).
This technique is able to cover multiple regions, including CpG islands, promoters and enhancer elements, and can yield data at a single-base resolution.
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