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Could authors incorporate an additional state to their model accounting for the possibility of multiple enhancer interactions?
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Each promoter may have multiple enhancer-promoter interactions, and thus may have more than one type of interactions.
This super-enhancer comprises multiple enhancer modules with selective activity that recruits a compendium of transcription factors, including GFI1b, RUNX1 and MYB.
A recent study showed that multiple enhancer variants cooperatively contribute to alter the expression of their gene targets58.
Self-inactivating (SIN) vector design reduced but did not abolish enhancer interactions.
Enhancer interactions may be involved in the regulation of eve stripe 7.
Lastly, genes are frequently regulated by multiple enhancer elements or clusters of enhancer elements.
As expected [ 23], a number of enhancer:enhancer interactions were also observed (N = 1209).
This in turn allows the extraction of all interactions that are between pairs of promoters (promoter:promoter interactions), or between other features of interest (e.g. promoter:enhancer or enhancer:enhancer interactions).
One such interaction overlaps the ß-catenin/TCF4 enhancer elements A through E. It is therefore possible that through ternary interactions, multiple enhancers are concurrently juxtaposed to MYC.
And studies in Drosophila suggest, too, that interactions of multiple enhancers are commonplace. .
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