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First, most of the features are conserved TF binding sites, while many enhancer elements are not conserved.
Many enhancer elements are located within 100 kilobases (kb) of the genes that they regulate, but can also be located more than a megabase away, or even on separate chromosomes.
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The stringent criteria for conservation applied in the two studies miss many known enhancer elements that are shorter than 200 bp, and highly conserved across all vertebrates.
Numerous NCCSs occur within mammalian genomes [ 39, 40], and while the majority of these have no known function, many do act as enhancer elements [ 41- 43].
We use coregulation of enhancer elements across many distinct regions of the brain to uncover functionally distinct networks at high resolution and link these networks to specific neuroglial functions.
HIFs transactivate the hypoxia-responsive element (HRE) present in the promoter or enhancer elements of many genes encoding angiogenic, metabolic and metastatic factors [ 3, 9, 10].
Although the intervals mapped with DamID do not correspond directly to enhancer elements, in many cases visual inspection reveals a very good correlation between annotated CRMs and peaks of DamID binding (e.g. dpp, Figure 2C).
RUNX1, also called AML1, encodes the alpha subunit of the core binding factor (CBF), which binds to the core element of many enhancer and promoter DNA regions.
Although this most often results in high-level transgene expression, the use of native cellular promoters or enhancer elements would be preferable in many circumstances.
Many experimentally verified functional elements, such as vista enhancer elements and transcription factor ChIP sequences, have been reported for human.
For many of these lines, the expression of Flp is controlled by local enhancer elements, thus allowing for tissue and cell-type-specific mitotic recombination.
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