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Small tumor fragments (∼1 mm3, estimated using 1 mm×1 mm grid) from a donor mouse were implanted into the right cerebral cortex of anesthetized recipient mice.

To better simulate the full metastatic cascade, we used an orthotopic graft model in which tumour sections, derived from tumours grown subcutaneously in a different mouse, were implanted on the stomach wall.

Small CT-2A tumour pieces (about 1 mm) from a C57BL/6J donor mouse were implanted into the right cerebral hemisphere of anaesthetised recipient mice as we recently described (Ranes et al, 2001).

Exponentially growing MX-1 cells (2 × 10 cells per mouse) were implanted in a 1 : 1 mix of growth factor reduced, basement membrane Matrigel matrix (BD Biosciences, Oxford, UK), and medium.

The P3 and P13 spheroids were stereotactically implanted into the brains of nude rats (n = 28 for P3 and n = 11 for P13) as well as in eGFP NOD/SCID mice (n = 5 for P3) as described previously [ 16, 40, 58] Briefly, GBM spheroids (10 per rat, 6 per mouse) were implanted into the right cerebral cortex using a Hamilton syringe fitted with a needle (Hamilton, Bonaduz, Switzerland).

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These were randomized so that each mouse was implanted with two different matrigel preparations (e.g. one from the negative control preparation and one containing normoxic mASCs).

Each athymic mouse was implanted subcutaneous with a 0.72-mg of 17-β-estradiol pellets, 2 weeks before inoculation of MCF-7 cells [17], [18].

Since each mouse was implanted with two xenografts, each group had 20 tumors.

Each BALB/c mouse was implanted intradermally on the ventral surface with the cells.

Each mouse was implanted with bilateral optic fibers, and stimulation was delivered either to one side ('unilateral #1'), the contralateral side ('unilateral #2') or to both sides ('bilateral'bilateral

To supplement the estrogens for MCF-7 proliferation each nude mouse was implanted with a 1.5 mg of 17β-estradiol pellet (Innovative Research of America, Sarasota, FL, USA).

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