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Coding sequences (CDS) of human and mouse were extracted from RefSeq transcripts.
The 14024 one-to-one orthologous gene relationships between human and mouse were extracted from Ensembl database via the Biomart.
Nucleotide sequences of 12 405 pairs of orthologous coding regions of human and mouse were extracted from the Searchable Prototype Experimental Evolutionary Database (SPEED) (http://www.bioinfobase.umkc.edu/speed/), using an in-house program developed in Perl.
All AS genes in cow which have alternatively spliced orthologues in both human and mouse were extracted to minimize any gene structure bias and to get the best-annotated genes in cow for analysis.
To minimize any gene structure bias and to get the best-annotated genes in chicken for analysis, an orthologous set of genes has been used, whereby all AS genes in chicken, which have alternatively spliced orthologous in both human, and mouse were extracted.
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Briefly, small portion of liver of all mice were extracted by treated with o-toluidine-glucose and after extraction; coupling reaction was done with trichloroacetic acid (TCA) and precipitation by alcohol and hydrolysis.
Whole breast tissues from MMTV-PyMT and wild-type (WT) control mice were extracted and analyzed for IL-25 expression.
The urine of adult female mice were extracted with dichloromethane (1 1 ratio, v/v) and analyzed by gas chromatography linked mass-spectrometry (GC MS).
To investigate the safety of PEGylated Au DENPs ([Au] = 0.1 M, 100 μL) in ApoE-/ mice and also the impact of the particles on the tissue structures, the major organs of the mice were extracted after 6 h post-injection of the PEGylated Au DENPs.
Therefore, incisors from cKO mice were extracted, paraffin embedded and processed for amelogenin immunostaining (Fig. 6).
Mandibules from wild-type and periostin−/− mice were extracted, decalcified with 10% EDTA for 1 month and embedded in paraffin.
Related(20)
rat were extracted
mouse were detected
mouse were removed
image were extracted
mouse were calculated
mouse were collected
rats were extracted
mouse were released
mouse were processed
mouse were proposed
mouse were confirmed
mouse were considered
mouse were taken
mouse were recorded
mouse were analyzed
mouse were swabbed
mouse were allowed
mouse were treated
mouse were obtained
mouse were harvested
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