mouse lymphoma cell line.
Adherent mouse fibroblasts, mouse lymphoma cell line RMA, spleen cells and dendritic cells were permeabilized with streptolysin O, and chicken ovalbumin (OVA), a model protein antigen, was successfully introduced into the cells.
Trifluorothymidine (TFT), a thymidine analogue and the selection agent normally used in the MLA, and 4-nitroquinoline-1-oxide (4-NQO), a potent mutagen, were used to treat cells from two different Tk+/− mouse lymphoma cell cultures with different background MFs (approximately 112 and 305 × 10−6).
The parental BW5147.3 and the Thy-1 negative, DPM1-deficient, mouse lymphoma cell lines [24], [25] (ATCC, Rockville, MD) and EBV-transformed cell lines were cultivated in RPMI 1640 Glutamax™ medium containing 10% fetal calf serum and 1% penicillin/streptomycin at 37°C under an atmosphere containing 5% CO2.
The mouse lymphoma cell lines YAC-1 (H2andand RMA (H2b) were maintained in NaHCO3-buffered Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FCS (Biochrom, Berlin, Germany), 2 mM L-glutamine, 1 mM sodium pyruvate, 100 U/ml penicillin, and 100 µg/ml streptomycin.
In order to evaluate fOSGN2-P generation in lymphoblastoid cells incapable of generating mature DLO, the DPM synthase deficient (null mutation in mouse DPM1 gene; see Fig. 1) mouse lymphoma cell line Thy-1 [37], [37] along with its parental cell line (BW5147.3) were examined as described above.
The inhibition of the IRP1 aconitase activity in L5178Y mouse lymphoma cells can increase "labile iron pool" levels.
It detects various mutation events involving the thymidine kinase (Tk) gene in L5178Y/Tk+/− −3.7.2C mouse lymphoma cells.
Both compounds showed no activity when evaluated for their cytotoxicity against L5178Y mouse lymphoma cells and no antibacterial activity against a broad spectrum of bacterial strains up to a concentration of 64 μg/mL [13].
Although previous in vitro cytotoxicity assays had showed that the CH2Cl2 extract of the fruits of the plant exhibited significant cytotoxicity (IC50 = 9.0 µg/mL) against P-388 mouse lymphoma cells [151, 152], the study by El-Gamal rather showed that the isolated compounds (218, 219 and 221 224) only showed marginal activity, with respective IC50 values of 60, 65, 42, 58, 94 and 88 µg/mL [149].
Here we demonstrate the presence of fOSGN2-P in EBV lymphoblastoid and mouse lymphoma cells.
