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This identified a 1bp deletion in exon 6 in the most repressed gene (At4g38360; Figure 3A) resulting in a frameshift and a premature stop (Figure S3A).

The most repressed gene was amt (12.5 folds).

The most repressed gene was GmPHP2, whose expression was reduced by 7.5-fold in shoots upon dehydration (Fig.  2B, Supplementary Fig. S2B, Supplementary Table S3).

We checked the differential expression of the thrombospondin gene (THBS1) that was found to be the most repressed gene in porcine PBMCs stimulated for 24 hours by PMA-Ionomycin [ 18].

Indeed, the second most repressed gene in the experiment with a −3.254 fold change was involved in arachidonic acid signalling i.e. potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2) [ 30].

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Table 4 The 10 most repressed genes by treatment with 83 μA/cm2 DC.

In addition, we noted that 18 of the 25 most induced genes and 17 of the 20 most repressed genes during villous CTB differentiation are TFAP2A-dependent [25].

Five genes (putatively) encoding bHLH transcription factors were among the 5% most repressed genes in glu1-2 leaves.

The genes LTB and FPR1 were both found in the top ten most repressed genes at T4 and T24 post stimulation.

The promoter sequences (-750, +250 with respect to the Transcription Start Site) of the 30 most induced and most repressed genes in shGATA3 cells at 48 h were studied using the MatInspector promoter analysis tool [ 13].

Interestingly, 2 of the 4 most repressed genes across all 5 KOs, Slc12a7, encoding a K/Cl− symporter, and Mcoln3 (also called Trpm3), are usually very weakly expressed in neuronal tissues, but strongly expressed in other tissues.

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