Sentence examples for most reliable gene from inspiring English sources
In this study, we used different combinations of reference genes in order to determine the most reliable gene combination.
The stability of each M. incognita gene expression during rice infection was analyzed using RefFinder Xieetet al. [2012]; http://www.leonxie.com/referencegene.php) and the most reliable gene was selected to normalise RT-qPCR data on M. incognita gene expression analysis in rice tissues.
Being conservative, we used the highest classification stringency to extract the most reliable gene groups.
Since we had amplified the full-length of pfsr12 by RT-PCR and sequenced it, we were certain about its intron-exon gene structure; with this knowledge in conjunction with analysis using FGENESH+ and the algorithm for homology comparison 'BLAST 2 sequences', we were able to identify the most reliable gene predictions.
qBasePlus corresponded with geNorm, evaluating EF1α as the most reliable gene in line Mitchell but differed in line V30, recommending ACT as the best candidate.
Consequently these were considered likely to contain the most reliable gene predictions and further analyses were focussed on this set of 17108 gene models from the rice Os_CD database.
The stability of each M. incognita gene expression across samples was analyzed using RefFinder Xieetet al. [2012]) in order to select the most reliable genes for using them as M. incognita reference genes for qPCR gene expression analysis in rice tissues.
Intron coordinates were taken from the Known Genes track of the UCSC Table Browser, which stores the most reliable gene-model data from the Uniprot and Refseq databases.
To select from the data base the most reliable genes, we first assessed reproducibility by testing the level of concordance in 14 reference experiments.
Additionally, AXIN2, the most reliable endogenous gene target of Wnt canonical pathway [ 28] activation was markedly upregulated upon mRNA analysis of high-grade breast tumours [ 7, 27].
Interestingly, sar1, encoding a small protein involved in the secretory pathway, which was recommended as the most reliable housekeeping gene [ 7] exhibited > 2-fold upregulation on wheat straw, probably because of the general upregulation of secretory genes under these conditions.
