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Exact(6)
The selection of the most appropriate gene for barcoding animals had been achieved several years earlier, but a botanical equivalent proved troublesome.
For each pair of profiles, we only included those probe sets that are the most appropriate gene representatives, and they must be "meaningfully" changed (p<0.05, and fold change>1.2) in at least one of the profiles.
In the remainder, the most appropriate gene was tested with negative results.
These two genes were also manually considered to be the most appropriate gene candidates for collagen-induced arthritis within this QTL.
β2-microglobulin (B2M), which was previously validated in our laboratory as the most appropriate gene for normalizing the data [ 12, 13, 42], was used as a housekeeping gene.
IFNG was rated as the top candidate by the CGC application and it was also considered to be the most appropriate gene candidate for collagen-induced arthritis within this QTL according to the manual inspection.
Similar(54)
We measured 7 housekeeping genes on the low-density arrays and applied Genorm [ 31] to determine the most appropriate genes for normalizing the results.
We applied geNorm [ 55] to the seven endogenous control genes on the LDAs to determine the most appropriate genes for normalizing the fold-change results.
Herein, we examine the evolutionary and biogeographic history of Pteronotropis using both sequences of one of the most appropriate genes for the scale of diversity being examined, ND2.
Our analyses of different floral organs revealed that GhACT4 and GhFBX6 are the most appropriated genes for qPCR normalization, since they represent the best combination of genes considered by NormFinder to improve NF.
Several statistical programmes can help to determine the most appropriate reference gene or set of genes.
Related(20)
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