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As a result, P. aeruginosa can 'shed' its mutator phenotype once it has acquired one or more adaptive mutations that serve to protect it from ROS.

In the Drosophila species studied, branch point enzymes accumulate more adaptive mutations than enzymes at the top of the pathway, which are conserved by strong purifying selection [ 10].

The conifer divergence was dramatically slower at synonymous sites than at non-synonymous sites (11-fold vs. 4-fold), suggesting that more adaptive mutations (and deleterious mutations, but see below) are fixed in conifers than in angiosperms.

There were significantly more adaptive mutations in endpoint populations of the control/benign treatments relative to those in the harsh treatments (U3,6 = 17, P = 0.039; Tables 2 and 3).

Considering that improvement of complex traits such as n-butanol tolerance might require accumulation of much more adaptive mutations [ 31, 32], the pQ-lib carrying cells were transferred in each n-butanol concentrations for three times, upon full growth, before transferring to the next higher butanol concentration.

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For comparison we also examined whether IS persistence is still favoured by more IS-induced adaptive mutations in the absence of bursts in transposition rates (Fig. 6, bottom panels, thick grey).

Besides, viruses with robust polymerase complexes might be more capable of creating adaptive mutations under a selection pressure.

As a result of their elevated spontaneous mutation frequencies, these strains are proposed to acquire adaptive mutations more frequently than those that are MMR-proficient [42], [54], [55].

Viruses with this specific PB2-PB1 combination were also found to be more capable to generate adaptive mutations under a new selection pressure.

While our results recapitulate the well-known conclusion that proteins affecting immunity and genome defense pathways are more likely to fix adaptive mutations, we also find that proteins affecting a suite of cell signaling pathways that are important for metazoan development are also fixing adaptive mutations in the D. melanogaster lineage at a significantly higher rate than the genome average.

The molecular adaptations include adaptive mutations and adaptive epigenetic modifications.

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