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Exact(5)
The BLAST results were filtered out using a Perl script according to criteria: a minimum nucleotide identity rate >90% and query coverage >80%.
In this step, a Helitron family was defined by E < e 6, pair-wise length >80 bp, and a minimum nucleotide identity rate of >80%.
A copy for Spy family was defined by an e value less than e−, length larger than 50 bp, and a minimum nucleotide identity of 80%.
The overlapping regions of fully sequenced BACs were determined using Sequencher 4.1.4 (GeneCodes) with 10% minimum overlap and 80% minimum nucleotide identity.
The 454 sequences (table 2) were first used to survey the presence of highly conserved reads of TR-GAG, using the criteria of 80% minimum nucleotide identity with over 80% of the read length.
Similar(55)
All subsequent reads were considered to be valid DNA or valid putative mRNA derived sequences and were annotated against the SEED database using MG-RAST (e-value <1×10−3; minimum length of alignment of 50 bp; minimum sequence nucleotide identity of 50%; [30]) to produce an abundance matrix of functional genes and protein-derived predicted taxonomies across the DNA and mRNA samples.
A minimum percent nucleotide identity threshold of 70% and 20 bp as a minimal length criterion were considered significant in VISTA [ 18] for our analyses.
In a study from Korea, the minimum polymerase nucleotide identity between strains related to the NB-like viruses was 80.9% (84.5% aa), while these strains presented a maximum of 78.4% nt (82.8% aa) identity with NA1-like strains (7 ).
For this analysis, blocks were defined as contiguous regions of 50 bp to 100 kbp displaying a minimum of 70% nucleotide identity.
Having found a minimum level of nucleotide identity of 98%% between two sequences within each TRAV subgroup, we have assumed that sequences with nucleotide identities ≥98%% represent allelic variants of genes; whilst, sequences with a level of identity <98% % correspond to distinct V genes.
All identified full-length LTR retrotransposons were clustered into families or group using the CD-HIT software [ 73] with a minimum of 70%% of nucleotide identity and a minimum sequence coverage of 70%% between related elements.
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