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One implementation of the algorithm clusters the sequences into the first cluster for which the minimum sequence identity threshold is satisfied.
The sequenced draft genome of H. parasuis serovar 5 strain 29755 (NZ_ABKM00000000) was aligned to the complete genome of H. parasuis strain SH0165 (CP001321) using BLASTN (minimum sequence identity of 95% and expected threshold of 1e−5).
The homology between our data and the Qin et al. [17] contig set was obtained using BLAT with 80% of the possible maximum alignment and a minimum sequence identity of 90%.
Based upon sequence similarity over the length of kinase domains (File S1), 993 Ser/Thr kinases from 303 prokaryotic genomes have been clustered into 270 clusters (File S2) with minimum sequence identity of 40% within a cluster.
NAHR-mediated events were inferred from a search of homologous blocks in the areas around the breakpoints (+/−500 bp) of minimum length of 30 bp and minimum sequence identity of 85%.
For UniGenes from T. aestivum, the minimum sequence identity requirement was increased to 95%.
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Minimum sequence identities above the 40%to70%0% level have been recommended by various researchers (Devos and Valencia, 2000; Friedberg, 2006; Lee et al., 2006; Rost, 2002).
The best blastx hits (E ≤ 1-10, minimum 30% sequence identity, minimum 30% of query aligned) to A. lyrata, E. parvulum, and RefSeq plants were recorded for all 27,016 genes.
A semi-automated bioinformatic pipeline was created using Perl to compare FC and BR fragment sequence clusters for each specimen using BLAST (blastn, megablast) requiring a minimum 98% sequence identity for each high-scoring segment pair (HSP), a minimum HSP length of 25 bp, with no more than 2 alignment mismatches.
All subsequent reads were considered to be valid DNA or valid putative mRNA derived sequences and were annotated against the SEED database using MG-RAST (e-value <1×10−3; minimum length of alignment of 50 bp; minimum sequence nucleotide identity of 50%; [30]) to produce an abundance matrix of functional genes and protein-derived predicted taxonomies across the DNA and mRNA samples.
Transcript assemblies (TA) are constructed using the TIGR Gene Indices Clustering TGICLL) pipeline (3) with a minimum sequence overlap identity between two sequences of 95%, a minimum overlap between sequences of 50 bp and a maximum overhang of non-overlapping sequences of 20 bp.
More suggestions(15)
minimum nucleotide identity
minimum sequence complementarity
minimum sequence match
minimum sequence coverage
minimum score identity
minimum sequence size
minimum sequence depth
minimum sequence length
minimum alignment identity
minimum sequence difference
minimum sequence threshold
minimum percent identity
minimum sequence separation
minimum mean identity
minimum sequence complexity
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