Sentence examples for minimum dna identity from inspiring English sources

Each new sequence was automatically validated as an extension of the initial fragment using the Cap contig assembly program included in the BioEdit software [ 68] with a minimum overlap between the two sequences of 50 bp, and a minimum DNA identity of 95%.

These IS share 86% DNA identity.

We then moved to a lower DNA identity level, recombining GFP and mRFP (45% DNA identity).

RNASE5 also shows homology to the LOC783225 angiogenin-1-like gene (83.6% DNA identity) and angiogenin 2 (75.8% DNA identity).

This approach combined with BLAST analyses (http://blast.ncbi.nlm.nih.gov/Blast.cgi) allowed us to determine that the minimum identity at the DNA level between a T3E on the membrane and a T3E ortholog in the probe had to be at least 71% to give a signal above background.

Parameters established for blast were: minimum identity: 25%; minimum ratio avec query: 60%; minimum ratio avec target: 50%.

The percentage of unique read hits to these contigs was determined using BLAT, with minimum sequence identity set to 70%, to find hits to the contigs of putative DNA transposon or retrotransposon origin.

To accommodate possible biases of this read mapping approach, additional DNA and RNA-seq read mappings were performed with CLC Genomics Workbench v 6.5 (mapping parameters: minimum 90% of read aligned and minimum alignment identity 95%), and resulting polymorphisms compared.

The pairwise nucleotide identity across all sequences in each set is roughly normally distributed around mean 52.8% (minimum mean identity 35.3%, maximum mean identity 93.9%, standard deviation 8%).

Clustering was performed using the following variable parameters: minimum percent identity for overlaps (94%), minimum overlap length (30 nt) and maximum length of unmatched overhangs (30 nt).

These parameters include a minimum overlap of 40 bp and a minimum overlap identity of 90%.