Sentence examples for min with intermittent from inspiring English sources

The tubes were then incubated at 70 °C for 30 min with intermittent mixing.

Tea sample (0.2 g, dry weight) was extracted with 5 ml 70%% methanol in a water bath at 70 °C for 10 min with intermittent shaking.

After homogenization, 5 μl RNase solutions (Conc. 10 mg/ml) was added, mixed five to six times by inverting the vial and incubated at 65 °C for 10 min with intermittent mixing.

The lipid films were hydrated with PBS (137 mM NaCl, 3 mM KCl, 6 mM Na2HPO4, 2 mM KH2PO4, pH 7.4) and allowed to rehydrate at 37°C for 30 min with intermittent swirling.

The tube was incubated on ice for 30 min with intermittent mixing and finally centrifuged for 5 min in a microfuge at 4°C, and the supernatant (nuclear extract) collected.

The nuclear pellet was resuspended in 30 µl nuclear extraction buffer containing 0.4 M KCl, 5 mM 2-mercaptoethanol and protease inhibitor cocktail (1∶100 dilution, Sigma) in STM buffer, and incubated on ice for 30 min with intermittent shaking and then centrifuged at 14,000×g for 20 min at 4°C.

Three milliliters of 2 1 chloroform/methanol was then added and the cells vortexed five times for 1 min each, with intermittent cooling in an ice bath.

After blocking endogenous peroxidase activity and nonspecific staining, the slides were incubated sequentially with primary antibodies (overnight at RT), antibody conjugate, and avidin-biotin complex at 37°C for 30 min, each with intermittent washing.

Third, the use of 15 min contracts coincides with intermittent power generation, as it incentivizes renewable energy providers to offer additional electricity.

Kidneys were dissected and dissociated in Hank's balanced salt solution (HBSS) containing 2.0 mg/mL collagenase IV (Sigma-Aldrich, St . Louis MO) and 200 μg/mL DNase I (Sigma) for 30 min at 37 °C with intermittent agitation.

HCl +10 ml methanol +38 ml H2O) for 15 min at 50°C with intermittent gentle shaking, before decanting and re-suspending in a neutralisation solution (7% NaOH in 60% ethanol) for 15 min at RT. Solutions were discarded and retained samples were rehydrated using a series of decreasing concentrations of ethanol (from 40, 20 and 10% v/v).