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Dynamic microPET scans over 120 min were acquired using a Siemens Concorde Focus 120 microPET (Siemens, Munich, Germany) [6] and followed by transmission measurement with 57Co point source.

Non-localised single-pulse 19F-MRS sequences (Block pulse centered at 209 MHz with 14 kHz bandwidth, FA 90°, TR 20 s, 100 excitations, total acquisition time 33 min) were acquired on Cell Sense-labelled cells, Cell Sense phantom and cell negative control to ensure the detectability of each sample.

Typically, 5 frames/s for the first 20 s, 2 frames/s for the next 30 s and 1 frame/s for the rest of ∼2 min were acquired.

For each participant, a total of 120 volumes during 4.68 min were acquired.

For the functional scan, whole-head T2∗-weighted echo-planar images (TR = 3s; TE = 50 ms; 120 volumes, 6 min), were acquired.

Stacks of typically 512 × 512 pixels × 3-6 optical sections (xyzt sampling: 0.28 × 0.28 × 2.5 μm × 1.5 5 min) were acquired for 4 10 hr, with Hoechst 33342 (Sigma) as vital DNA dye.

Approximately 45 min post-injection, a static 3D emission scan of 15 min was acquired.

A 2D encoded T1-weighted FSE sequence with increased bandwidth (TR 339 ms, TE 13.6 ms, slice thickness 3 mm, receiver bandwidth 142.86 kHz, acceleration factor of 1.75, acquisition time of 3.16 min) was acquired in axial orientation.

A free-breathing black blood fast spin echo (FSE) T1w sequence with an acquisition time of 3 4 min is acquired in four identical axial slices, both before and after (without any change in the parameters in between) intravascular injection of 0.1 mmol of gadopentetate dimeglumine (Gd-DTPA) [11].

At 30 min after injection, the first SPECT scan of 45 min was acquired.

A baseline 256-acquisition GABA-optimized spectrum lasting approximately 15 min was acquired prior to stimulation, and a 384-acquisition GABA-optimized spectrum lasting approximately 20 min was acquired immediately after stimulation.